This is a knockout-validated antibody summary, based on the publications "Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion", as cited below [1], and "PKN1 Is a Novel Regulator of Hippocampal GluA1 Levels" for western blot knockout validation (figure 2a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: BD Biosciences
Antibody: PRK1
Catalog number: 610686
Summary: Mouse monoclonal IgG1 against human PRK1 aa. 215-388. Reacts with human, dog, rat, and mouse. Suitable for western blot, immunofluorescence, and immunoprecipitation. Not recommended for immunohistochemistry.
Western blot
WT and PKN1 knockout (-/-) mouse embryo extracts.
3% BSA in TBS containing 0.1% Tween 20.
Appropriate HRP-conjugated secondary antibodies (Amersham).
ECL and Image Quant digital imaging system (GE healthcare) or X-ray film.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.