This is a knockout-validated antibody summary, based on the publication "Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of PRDX6 in WT and PRDX6-knockout PC9 cells. β-actin was used as loading control. From [1].

Rabbit polyclonal IgG

Company: ProteinTech

Antibody: PRDX6

Catalog number: 13585-1-AP

Summary: Rabbit polyclonal IgG against a human PRDX6 fusion protein. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry and ELISA.

Western blot

WT and PRDX6 knockout PC9 cells. Cell lysates were prepared using RIPA lysis buffer (Thermo Scientific) containing a protease inhibitor cocktail (Thermo Scientific).

1:1,000 dilution.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.