This is a knockout-validated antibody summary, based on the publication "Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot performed on PRDM8-KO and control VN-MCC cell lines. β-actin was used as an internal positive control. From [1].

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: PRDM8

Catalog number: sc-390001

Summary: Mouse monoclonal antibody against amino acids 1-125 mapping at the N-terminus of human PRDM8. Recommended for detection of PRDM8 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Control and PRDM8-KO VN-MCC cell lines.

Odyssey blocking buffer (LI-COR, Lincoln, NE, USA) for 1 hour at room temperature (RT).

Overnight at 4 C.

IRDye 800 secondary antibodies (LI-COR) for 1 hour at RT.

Odyssey Imaging System (LI-COR).