This is a knockout-validated antibody summary, based on the publication "Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal IgG

Company: Bethyl

Antibody: PPP6C

Catalog number: A300-844A

Summary: Rabbit polyclonal IgG against a synthetic peptide corresponding to residues between 250 and C-term of human PPP6C. Reacts with human and mouse by western blot and immunoprecipitation. It is predicted to react with additional species including rat, zebrafish, X. laevis, chicken, bovine, dog, monkey and horse.

Western blot | Immunocytochemistry

Oocytes from control Ppp6F/F and and Ppp6F/F;ZCre+ mice. WB: oocytes were directly lysed in 2× SDS sample buffer. ICC: Oocytes were fixed in 4% paraformaldehyde in PBS for 30 min at room temperature.

WB: TBST containing 5% skimmed milk, for 1 hour at room temperature.

ICC: 1% BSA-supplemented PBS for 1 h.

WB/ICC: Overnight at 4°C

WB: 1∶1,000 HRP-conjugated goat anti-rabbit IgG (ZhongShan Golden Bridge Biotechnology Co., Beijing, China) for 2 hours at room temperature.

WB: enhanced chemiLuminescence detection system (Bio-Rad, CA).

ICC: Laser scanning confocal microscope (Zeiss LSM 780 META, Germany).

Please see Figure 2a,b in the article [1].