PPP2R2A antibody | knockout validation | Cell Signalling 5689

This is a knockout-validated antibody summary, based on the publication "IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PPP2R2A antibody | knockout validation | Cell Signalling 5689 figure 1
Figure 1. Western blot showing IER5 and B55a protein levels in single (KO) and double (DKO) PPP2R2A and IER5 knockout clones in the presence of GSI (-) and 4 hr after GSI washout (+). From [1].
Antibody information

Mouse monoclonal IgG1

Company: Cell Signalling

Antibody: PPP2R2A

Catalog number: 5689

Summary: Mouse monoclonal antibody against a synthetic peptide corresponding to residues surrounding Asp406 of rat PP2R2A/PP2A, B55-α/PR55-α protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and PPP2R2A knockout SCC cells.

Primary incubation

1:1,000 dilution.

Detection

Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific).

Clone note

The same clone (2G9) is sold as BioLegend 831301, 831303, 831304; MilliporeSigma SAB4200241; Santa Cruz Biotechnology sc-81606; Enzo Life Sciences ALX-804-569-C100; Cell Signaling Technology 5689.

References
  1. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher