This is a knockout-validated antibody summary, based on the publication "Loss of PPARγ expression by fibroblasts enhances dermal wound closure", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Indirect immunofluorescence analysis of WT (C/C) and PPARg KO (K/K) mice skin samples (original magnification × 40, bar = 25 um) using an anti- PPARg antibody. Red = PPAR gamma; Blue = DAPI. From [1].

Western blot

Antibody: PPARg

Catalog number: sc-7196

Summary: Rabbit polyclonal IgG raised against epitope corresponding to amino acids 8-106 mapping at the N-terminus of PPARg1 of human origin. Recommended for detection of PPARg1 and PPARg2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA; also reactive with additional species, including equine, canine and porcine.

Company: Santa Cruz Biotechnology

Immunohistochemistry, IHC

Skin sections from mice homozygous for loxP-PPARg and hemizygous for an allele enabling a tamoxifen-dependent cre recombinase to be expressed under the control of a fibroblast-specific collagen type I promoter/enhancer.

BSA (0.1%) in PBS for 1 hour

1:500 in a humidified chamber overnight at 4C

1:400 Dylight 594-conjugated donkey anti-rabbit (711-485-152, Jackson Immunoresearch) for 1 hour.

In the article, IHC is done with this antibody. Western blot is done with one antibody from the same company, but there is no detailed information about the PPARg antibody used for Western blot. Presumably it is the same antibody.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.