PPARalpha antibody | knockout validation | Cayman Chemical Cay101710-1
DOI
//dx.doi.org/10.13070/ko.en.7.2209
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "Glucocorticoid receptor-PPARα axis in fetal mouse liver prepares neonates for milk lipid catabolism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PPARalpha antibody | knockout validation | Cayman Chemical Cay101710-1 figure 1
Figure 1. Immunoblot of PPARα in Ppara-/- and Ppara+/+ liver nuclear extracts. U2AF65 was used as loading controls. From [1].
Antibody information

Rabbit polyclonal IgG

Company: Cayman Chemical

Antibody: PPARalpha

Catalog number: Cay101710-1

Summary: Rabbit polyclonal IgG against amino acids 22-36 of human PPARalpha. Reacts with human, mouse and rat by western blot.

Validation Method

Western blot

Sample

Liver nuclear protein fractions from Ppara-/- and Ppara+/+ mice.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX).

Detection

Chemiluminescence was detected using the Luminata Crescendo Western HRP Substrate (Millipore, MA).

References
  1. Rando G, Tan C, Khaled N, Montagner A, Leuenberger N, Bertrand-Michel J, et al. Glucocorticoid receptor-PPARα axis in fetal mouse liver prepares neonates for milk lipid catabolism. elife. 2016;5: pubmed publisher