PPAR alpha antibody | knockout validation | Abcam ab97609

This is a knockout-validated antibody summary, based on the publication "Peroxisome proliferator activated receptor alpha inhibits hepatocarcinogenesis through mediating NF-κB signaling pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PPAR alpha antibody | knockout validation | Abcam ab97609 figure 1
Figure 1. Immunoblot of PPARα in HCCs from PPARα-/- and WT mice. GAPDH was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Abcam

Antibody: PPAR alpha

Catalog number: ab97609

Summary: Mouse monoclonal IgG2a against a recombinant fragment corresponding to amino acids 4-96 of human PPAR alpha. Reacts with mouse and human. Suitable for western blot, ELISA, immunoprecipitation (IP), Chromatin IP, and EMSA.

Validation Method

Western blot

Sample

Hepatocellular carcinomas from PPARα-/- and WT mice.

Primary incubation

Overnight at 4°C.

Secondary incubation

For 1 hour at room temperature.

Detection

ECL Plus Western blotting Detection Reagents (GE Healthcare, Piscataway, NJ).

Clone note

The same clone (H0723) is sold as Abcam ab97609; R&D Systems PP-H0723-00.

References
  1. Zhang N, Chu E, Zhang J, Li X, Liang Q, Chen J, et al. Peroxisome proliferator activated receptor alpha inhibits hepatocarcinogenesis through mediating NF-κB signaling pathway. Oncotarget. 2014;5:8330-40 pubmed