This is a knockout-validated antibody summary, based on the publication "Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of PP5 in control and mutant mouse MEF cells homozygous for a null mutation in the gene encoding the PP5 catalytic subunit. LC are background bands used as loading control. From [1].

Mouse polyclonal

Company: Abnova

Antibody: PP5

Catalog number: H00005536-B01

Summary: Mouse polyclonal antibody raised against a full-length human PPP5C protein. Reacts with the human protein and it is suitable for western blot and immunofluorescence. For IHC and IF applications, antibody purification with protein A will be needed prior to use.

Western blot

Mouse MEF cells homozygous for a knockout allele of the PP5 gene.

Goat anti-mouse IgG (H+L)-HRP conjugate (catalog #170-6516; Bio-rad) at a 1:3,000 dilution.

ECL Western Blotting Substrate (Thermo Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.