This is a knockout-validated antibody summary, based on the publication "B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: PP2A A Subunit
Catalog number: 2041
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human PP2A A Subunit protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.
Western blot
Patient-derived bone marrow-derived B cell lymphoma xenograft cells. Cells were lysed in CelLytic buffer (Sigma-Aldrich) supplemented with 1% protease inhibitor cocktail (Fisher Scientific).
Western Breeze Immunodetection System (Life Technologies) was used for signal development, and chemiluminescent signal was detected by X-ray film exposure.
Patient-derived Ph+ ALL xenograft cells were used for doxycycline (Dox)-inducible CRISPR-Cas9-mediated gene disruption of PPP2R1A (3 different gRNAs were used). Western blot was performed to measure expression of PP2A and phosphorylation of H2AX after 3 days of Dox-treatment. Please see Figure 7a in the article [1].
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