This is a knockout-validated antibody summary, based on the publication "Zinc finger nuclease knock-out of NADPH:cytochrome P450 oxidoreductase (POR) in human tumor cell lines demonstrates that hypoxia-activated prodrugs differ in POR dependence", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: Santa Cruz

Antibody: POR

Catalog number: sc-25263

Summary: Mouse monoclonal IgG2a against amino acids 1-300 of human cytochrome P450 reductase. Recommended for detection of CYPOR (POR) of human origin by western blotting, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and POR knock-out HCT116 and SiHa cells.

1:2,500 dilution.

1:10,000 dilution goat anti-mouse secondary antibody (Santa Cruz Biotechnology, sc-2055).

SuperSignal West Pico chemiluminescent substrate.

Please see Figure 4b,e in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.