PML antibody | knockout validation | Millipore MAB3738

This is a knockout-validated antibody summary, based on the publication "A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PML antibody | knockout validation | Millipore MAB3738 figure 1
Figure 1. Pml expression in MSCs derived from Pml +/+ or Pml −/− mice is shown on the left. From [1].
Antibody information

Mouse monoclonal IgG2b

Company: Millipore

Antibody: PML

Catalog number: MAB3738

Summary: Mouse monoclonal IgG2b against PML fusion protein corresponding to amino acids 1-581 of mouse PML. Reacts with mouse by western blot, immunoprecipitation and immunocytochemistry.

Validation Method

Immunocytochemistry

Sample

Cells were fixed with 4% PFA for 10 min.

Blocking agent

PBS, Triton X-100 0.2 and 10% FBS for 30 min.

Primary incubation

Overnight in blocking buffer.

Secondary incubation

Anti-Mouse-564.

Clone note

The same clone (36.1-104) is sold as Abnova MAB3726; Enzo Life Sciences ALX-804-816-C100; Active Motif 39773; EMD Millipore MAB3738, 05-718.

References
  1. Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher