This is a knockout-validated antibody summary, based on the publication "TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Lysates from wild-type (WT) or PML knockout (KO) MEFs were immunoblotted and probed with anti-PML and anti-HSP90 (loading control). From [1].

Rabbit polyclonal IgG

Company: Bethyl-Laboratories

Antibody: PML

Catalog number: A301-167A

Summary: Rabbit polyclonal IgG against an epitope located between aa 375 and 425 of human PML. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence, and proximity ligation assay.

Western blot

PML knockout and wild-type murine embryonic fibroblasts (MEF) derived from C57BL6 mice. Cells were lysed in NP40 lysis buffer (10 mM TrisHCl (pH 7.5), 150 mM NaCl, 2 mM EDTA, 0.5% NP-40, Halt Protease Inhibitor).

Used in combination with A301-168A.

anti-rabbit horseradish peroxidase (HRP)-labeled secondary antibody (goat, GE Healthcare).

HRP substrate (Pierce, Rockford, IL, USA).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.