PMCA4 antibody | knockout validation | Abcam ab2783
DOI
//dx.doi.org/10.13070/ko.en.6.1775
Date
2016-11-07

This is a knockout-validated antibody summary, based on the publication " Calcium Extrusion Pump PMCA4: A New Player in Renal Calcium Handling?", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PMCA4 antibody | knockout validation | Abcam ab2783 figure 1
Figure 1. Immunofluorescent detection of PMCA4 using anti-PCMA antibody (abcam2783, clone JA9) in kidney samples from wild-type (B) and PMCA4 (-/-) (C) mice. From [1].
Antibody information

Mouse monoclonal

Company: Abcam

Antibody: PMCA4

Catalog number: Ab2783 (clone JA9)

Summary: Raised against purified human erythrocyte PMCA. Supplier recommended for Elisa, immunofluorescence, immunohistochemistry, flow cytometry and Western blotting.

Detects mouse, rat, rabbit, cow, pig and human PMCA4.

Validation Method

Immunofluorescence

Sample

Mouse kidney sections fixed with 1%(w/v) periodate-lysine-paraformaldehyde

Blocking agent

30 minutes in 0.15 M NaCl / 0.1 M Tris-HCl pH 7.5 containing 0.5% (w/v) NEN Life Sciences blocking reagent

Primary incubation

Overnight at 40C (1:100)

Secondary incubation

Alexa Fluor 488-conjugated goat anti-mouse antibody (Invitrogen) at 1:300 for 2 hours

Detection

AxioCam camera with Axiovision software (Zeiss)

References
  1. van Loon E, Little R, Prehar S, Bindels R, Cartwright E, Hoenderop J. Calcium Extrusion Pump PMCA4: A New Player in Renal Calcium Handling?. PLoS ONE. 2016;11:e0153483 pubmed publisher