This is a knockout-validated antibody summary, based on the publication "MARK2 phosphorylates eIF2α in response to proteotoxic stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. PERK, PKR, HRI, GCN2, and MARK2 proteins were analyzed by immunoblotting in the 4-KO and 5-KO MEFs as compared to the WT MEFs. From [1].

Mouse monoclonal IgG2a κ

Company: Santa Cruz

Antibody: PKR

Catalog number: sc-6282

Summary: Mouse monoclonal against amino acids 1-515 of mouse PKR. Recommended for detection of PKR of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Western blot

WT and PKR KO MEFs.

5% w/v BSA and 0.05% NaN3 in TBST.

At 4°C overnight

Odyssey imager and analyzed with Image Studio software (Licor 9120, USA).