This is a knockout-validated antibody summary, based on the publication "Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Company: R&D Systems
Antibody: PKN2
Catalog number: MAB5686
Summary: Mouse monoclonal IgG1 against recombinant human PKN2
Asp18-Asn207. Reacts with human, mouse, and rat by western blot.
Immunohistochemistry | Immunocytochemistry
IHC: WT and PKN2-KO mouse embryos. Embryos were embedded in OCT and frozen for sectioning. ICC: MEFs from WT and PKN2-KO.
2% BSA in PBS.
Alexa-488 secondary antibody.
Zeiss LSM confocal microscopy.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.