This is a knockout-validated antibody summary, based on the publication "Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blots indicate that endothelial PKN2 is deleted in Tie2-Cre PKN2fl/fl mice. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: PKN2

Catalog number: ab119936

Clone: 1D1. This clone is also sold as Invitrogen MA5-15887, Abnova MAB10301, EMD Millipore MABT496, and LifeSpan Biosciences LS-C169402.

Summary: Mouse monoclonal IgG1 against a recombinant fragment corresponding to amino acids 555-718 of human PKN2. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunohistochemistry (paraffin), ELISA and flow cytometry.

Western blot

Mouse lung endothelial cell lysates from WT and Tie2-Cre PKN2fl/fl mice.

3% BSA in TBS containing 0.1% Tween 20.

Appropriate HRP-conjugated secondary antibodies (Amersham).

ECL and Image Quant digital imaging system (GE healthcare) or X-ray film.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.