PKM2 antibody | knockout validation | Cell Signaling 4053

This is a knockout-validated antibody summary, based on the publications "Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury" (Western blot, figure 4h) [1] and "Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction", as cited below [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: PKM2

Catalog number: 4053

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human PKM2. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

WT (PKM2fl/fl) and PKM2-KO (PKM2fl/fl ; Podocin cre+) mouse kidney podocytes.

Primary incubation

1:1,000 dilution.

Figure

Representative blot images of PKM2 protein in podocytes isolated from WT and PPKM2-KO mice. Please see Figure 3f in the article [2].

References
  1. Zhou H, Zhang R, Anand P, Stomberski C, Qian Z, Hausladen A, et al. Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury. Nature. 2019;565:96-100 pubmed publisher
  2. Qi W, Keenan H, Li Q, Ishikado A, Kannt A, Sadowski T, et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med. 2017;23:753-762 pubmed publisher