This is a knockout-validated antibody summary, based on the publication "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot validating complete loss of PKCB protein expression in PKCB-knockout RBL‐2H3 cells. β-tubulin was used as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: PKCB

Catalog number: sc-13149

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 657-673 at the C-terminus of human PKCβII. Recommended for detection of cPKCβII of mouse, rat, and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA. Also reactive with additional species, including bovine and porcine.

Western blot

WT and PKCB-knockout RBL‐2H3 cells.