PKCβ antibody | knockout validation | BD Biosciences 610127

This is a knockout-validated antibody summary, based on the publication "PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PKCβ antibody | knockout validation | BD Biosciences 610127 figure 1
Figure 1. Immunoblot analysis of PKCβ in brains from neonatal mice double knockout of PKCα and PKCβ isoforms (DKO) prepared alongside control brains from wild-type littermates. Actin antibody loading control shown. DKO samples use the same Actin loading. From [1].
Antibody information

Mouse monoclonal IgG2b

Company: BD Biosciences

Antibody: PKCβ

Catalog number: 610127

Summary: Mouse monoclonal IgG2b against human PKCβ (aa. 126-324). Reacts with rat, human, mouse, and chicken. Suitable for western blot, immunoprecipitation, and immunofluorescence. Not recommended for immunohistochemistry.

Validation Method

Western blot

Sample

Wild-type and PKCα/PKCβ double knockout brains from neonatal mice.

Blocking agent

5% non-fat dry milk in TBS with 0.05% Tween 20.

Secondary incubation

1:5,000 dilution anti-mouse IgG secondary antibodies (Cell Signaling Technology, 7076S) in the same blocking buffer as the primary antibody.

References
  1. Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher