PKCα antibody | knockout validation | BD Biosciences 610107

This is a knockout-validated antibody summary, based on the publication "PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PKCα antibody | knockout validation | BD Biosciences 610107 figure 1
Figure 1. Immunoblot analysis PKCα in brains from neonatal mice double knockout of PKCα and PKCβ isoforms (DKO) prepared alongside control brains from wild-type littermates. Actin antibody loading control shown. DKO samples use the same Actin loading. From [1].
Antibody information

Mouse monoclonal IgG2b,κ

Company: BD Biosciences

Antibody: PKCα

Catalog number: 610107

Summary: Mouse monoclonal IgG2b,κ against a recombinant protein corresponding to human PKCα (aa. 270-427). Reacts with rat, human, mouse, dog, chicken, and frog. Suitable for western blot, immunohistochemistry, immunoprecipitation, and immunofluorescence.

Validation Method

Western blot

Sample

Wild-type and PKCα/PKCβ double knockout brains from neonatal mice.

Blocking agent

5% non-fat dry milk in TBS with 0.05% Tween 20.

Secondary incubation

1:5,000 dilution anti-mouse IgG secondary antibodies (Cell Signaling Technology, 7076S) in the same blocking buffer as the primary antibody.

References
  1. Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher