PKCε antibody | knockout validation | BD Biosciences 610085

This is a knockout-validated antibody summary, based on the publication "PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PKCε antibody | knockout validation | BD Biosciences 610085 figure 1
Figure 1. Immunoblot analysis of PKCε in brains from neonatal mice of PKCε isoform knockout (KO) prepared alongside control brains from wild-type littermates. Actin antibody loading control shown. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: BD Biosciences

Antibody: PKCε

Catalog number: 610085

Summary: Mouse monoclonal IgG2a against a recombinant protein corresponding to human PKCε (aa. 1-175). Reacts with rat, human, mouse, dog, and chicken. Suitable for western blot, immunohistochemistry, immunoprecipitation, and immunofluorescence.

Validation Method

Western blot

Sample

Wild-type and PKCε knockout brains from neonatal mice.

Blocking agent

5% non-fat dry milk in TBS with 0.05% Tween 20.

Secondary incubation

1:5,000 dilution anti-mouse IgG secondary antibodies (Cell Signaling Technology, 7076S) in the same blocking buffer as the primary antibody.

References
  1. Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher