This is a knockout-validated antibody summary, based on the publication "Hypothalamic PKA regulates leptin sensitivity and adiposity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blots of RIIβ in the hypothalamus of WT and RIIβ KO mice. Each lane of the blots represents a single mouse. From [1].

Company: BD Biosciences

Antibody: PKA RIIbeta

Catalog number: 610626

Summary: Mouse monoclonal IgG1 against human PKA RIIβ, aa 1-418. Reacts with human, mouse, rat, dog, and chicken . Suitable for western blot and immunofluorescence. Not recommended for immunoprecipitation or immunohistochemistry.

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Figure 2. Representative images of immunofluorescent staining of RIIβ in the hypothalamus of fasted WT (n = 3) and RIIβ KO (n = 3) mice. Scale bars, 50 µm. From [1].

Western blot | Immunohistochemistry

Hypothalamus of of WT and RIIβ KO mice. WB: The tissue was homogenized in 200 μl lysis buffer (50 mM Tris-Cl, pH7.4, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% deoxycholic acid) supplemented with protease and phosphatase inhibitors (5 mM NaF, 1 mM Na3VO4, PhosStop (Roche), cOmplete Ultra protease inhibitors (Roche)). IHC: Brains were removed and postfixed, followed by cryopreservation in 30% sucrose solution (w/v) overnight and subsequent freezing in OCT compound (Tissue-Tek).