PIK3R2 antibody | knockout validation | AbD Serotech MCA1170G

This is a knockout-validated antibody summary, based on the publication "Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PIK3R2 antibody | knockout validation | AbD Serotech MCA1170G figure 1
Figure 1. Western blot analysis of wild-type or PIK3R2 (p85) knockout HAP-1 cells. From [1].
Antibody information

Mouse monoclonal IgG1

Company: AbD Serotech

Antibody: PI-3 Kinase p85 Subunit Beta

Catalog number: MCA1170G

Summary: Mouse monoclonal IgG1 against recombinant p85 beta subunit of bovine PI-3 kinase. Reacts with human, monkey and bovine. Suitable for western blot, immunoprecipitation and immunohistochemistry (frozen).

Validation Method

Western blot

Sample

Wild-type or PIK3R2 (p85) knockout HAP-1 cells.

Secondary incubation

Fluorochrome-conjugated anti-mouse (ThermoFisher Scientific, SA5-10176).

Detection

LI-COR Odyssey Fc scanner.

Clone note

The same clone (T15) is sold as Bio-Rad MCA1170G; Santa Cruz Biotechnology sc-56934; LifeSpan Biosciences LS-C43327; Abcam ab28356; Invitrogen MA1-90048; MyBioSource MBS212045.

References
  1. Turkington H, Juozapaitis M, Tsolakos N, Corrales Aguilar E, Schwemmle M, Hale B. Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins. J Virol. 2018;92: pubmed publisher