This is a knockout-validated antibody summary, based on the publication "Protein Interacting C-Kinase 1 Modulates Surface Expression of P2Y6 Purinoreceptor, Actin Polymerization and Phagocytosis in Microglia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: NeuroMab
Antibody: PICK1
Catalog number: 73-040
Summary: Mouse monoclonal IgG2a against a fusion protein corresponding to aa 10-130 of human PICK1. Reacts with human, rat and mouse. Suitable for immunoblot, immunocytochemistry, immunohistochemistry and
immunoprecipitation.
Western blot
Microglial cells from wild-type and PICK1-knockout (KO) mice.
1:2,000 dilution.
1:10,000 dilution horseradish peroxidase-conjugated secondary antibodies (Thermo, Rockford, IL).
Enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL).
Western blot of PICK1 in microglial cells from WT and PICK1-KO mice. GAPDH was used as loading control. Please see Figure 2a in the article [1].
The same clone (L20/8) is sold as BioLegend 830901; Neuromab 73-040, 75-040.
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