This is a knockout-validated antibody summary, based on the publication "PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of whole-cell extracts from wild type, PICH+/− and two PICH−/− clones probed with a PICH-specific antibody. Purified recombinant human PICH (rPICH) is shown as control. β-Tubulin was used as a loading control. From [1].

Mouse monoclonal

Company: Abcam

Antibody: PICH

Catalog number: ab57434

Summary: Mouse monoclonal against recombinant full length protein, corresponding to amino acids 1-420 of human PICH. Suitable for the detection of human protein by western blot.

Western blot

PICH−/− DT40 (chicken) cell lines generated by targeted inactivation of both PICH alleles. Cells were snap frozen and lysed with cold RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS (Sigma)) supplemented with Complete EDTA free (Roche) on ice.

5% milk in PBS-T (Sigma)

1:500 in 5% milk in PBS-T

in 5% milk in PBS-T

ECL (Pierce)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.