This is a knockout-validated antibody summary, based on the publication "Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot for PICALM and actin (loading control) protein levels in representative Picalm-knockout and PICALM-rescued hematopoietic fetal liver cells. From [1].

Rabbit polyclonal

Company: Sigma-Aldrich

Antibody: PICALM

Catalog number: HPA019061

Summary: Rabbit polyclonal against a peptide (DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL) of human PICALM. Reacts with human, mouse, and rat and has been used in western blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Western blot

Hematopoietic fetal liver cells harvested from E14 fit1 Picalmfit1-5R mouse embryos

overnight in Odyssey Blocking Buffer (Li-Cor) with 0.1% Tween-20

in Odyssey Blocking Buffer with 0.1% Tween-20 and 0.02% SDS for 30 min

Odyssey Infrared fluorescence imaging system (Li-Cor)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.