This is a knockout-validated antibody summary, based on the publication "Requirement for class II phosphoinositide 3-kinase C2alpha in maintenance of glomerular structure and function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblotting with different anti-PI3KC2α (α-PI3KC2α) of rat, wild-type (+/+) mouse and PI3KC2α knockout (-/-) mouse tissue lysates. GAPDH is used as loading control. From figure 1 [1]. Ab #1, BD Biosciences; Ab #2, Santa Cruz Biotechnology; Ab #3, described in the article "The CC chemokine monocyte chemotactic peptide-1 activates both the class I p85/p110 phosphatidylinositol 3-kinase and the class II PI3K-C2alpha" [2].

Antibody: PI3KC2alpha

Catalog number: 611046

Summary: Mouse IgG1 raised against mouse PI3-Kinase p170. It reacts with rat, human, and mouse proteins and it has been used in western blot and immunofluorescence.

Validation Method: Western blot, WB

Antibody: PI3KC2alpha

Catalog number: sc-67306

Validation Method: Western blot, WB

Antibody: PI3KC2alpha

Catalog number: PIK3C2A-jw-9748276 (Labome VAD designation). The antibody is described in [2].

Validation Method: Western blot.

Cell lysates were prepared from brain and kidney tissues

HRP-conjugated secondary Abs with appropriate specificity

ECL detection system (GE Life Sciences, Pittsburgh, PA).

KO tissues expressed an ∼170-kDa protein corresponding to a PI3KC2α form that lacked a 267-amino-acid segment encompassing the C-terminal PX and C2 domains.