This is a knockout-validated antibody summary, based on the publication "A PI3K p110β-Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure S1 in [1]. Western blot of spleen lysates from wild-type and p110δ -/- mice probed with anti-p110δ antibody. Vinculin serves as a loading control.

Mouse monoclonal

IgG1 kappa

Company: Santa Cruz Biotechnology

Antibody: PI3K p110δ

Catalog number: sc-55589

Summary: Mouse monoclonal antibody raised against a polypeptide corresponding to residues 363-582 from the N-terminal region of human PI3K p110δ.

Western blot

Lysates of spleen from wild-type and PI3K p110δ -/- mice.

TBS containing 5% milk powder.

Anti-p110δ antibody diluted 1:1000.

LI-COR 680- conjugated anti-mouse IgG.

Odyssey detection system (LI-COR).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.