PHGDH antibody | knockout validation | Sigma-Aldrich WH0026227M1

This is a knockout-validated antibody summary, based on the publication "Rational Design of Selective Allosteric Inhibitors of PHGDH and Serine Synthesis with Anti-tumor Activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PHGDH antibody | knockout validation | Sigma-Aldrich WH0026227M1 figure 1
Figure 1. Western blot analysis of PHGDH in control and PHGDH KO SKOV3 cells, with beta-actin as a loading control. Courtesy from the Authors [1].
Antibody information

Mouse monoclonal IgG1κ

Company: Sigma-Aldrich

Antibody: PHGDH

Catalog number: WH0026227M1

Summary: Mouse monoclonal IgG1κ against full-length recombinant PHGDH protein. Reacts with human by western blot, immunoprecipitation , immunohistochemistry (paraffin), immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Control and PHGDH-KO SKOV3 cells. Protein was extracted from cells using 1x radioimmunoprecipitation assay buffer (Rockland Immunochemicals).

Blocking agent

5% dry milk in Tris-buffered saline with Tween 20.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:2,000 dilution horseradish peroxidase-conjugated anti-mouse antibody (Rockland Immunochemicals, 611G4302).

Detection

Chemiluminescent signals were detected with a Clarity Western ECL Detection Kit (Bio-Rad) and imaged using a ChemiDoc MP system (Bio-Rad).

Clone note

The same clone (4A3-1D6) is sold as LifeSpan Biosciences LS-B5457; Abnova H00026227-M01; Sigma-Aldrich WH0026227M1.

References
  1. Wang Q, Liberti M, Liu P, Deng X, Liu Y, Locasale J, et al. Rational Design of Selective Allosteric Inhibitors of PHGDH and Serine Synthesis with Anti-tumor Activity. Cell Chem Biol. 2016;: pubmed publisher