This is a knockout-validated antibody summary, based on the publications "Prolyl hydroxylase domain proteins regulate bone mass through their expression in osteoblasts", as cited below [1], and "Control of endothelial quiescence by FOXO-regulated metabolites" for western blot knockout validation (figure s10a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: PHD1/prolyl hydroxylase
Catalog number: ab113077
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human PHD1/prolyl hydroxylase. Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry, immunohistochemistry (paraffin) and immunoprecipitation. Unsuitable for flow cytometry.
Immunohistochemistry
WT and Phd1–3ob−/− mouse tibiae. Tissues were fixed in 10% formalin, decalcified and embedded in paraffin.
EnVision System HRP (Dako North America, Inc., Carpinteria, CA).
Immunohistochemical staining of femoral sections of 2-month-old control and Phd1–3ob−/− male mice with antibodies against PHD1, PHD2, PHD3 or normal IgG. Arrows indicate PHD-expressing osteoblasts located on trabecular surfaces. Please see Figure 1 in the article [1].
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