PHD-2/Egln1 antibody | knockout validation | Cell Signaling 4835

This is a knockout-validated antibody summary, based on the publication "Control of endothelial quiescence by FOXO-regulated metabolites", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PHD-2/Egln1 antibody | knockout validation | Cell Signaling 4835 figure 1
Figure 1. Immunoblot analysis of PHD1/2/3-depleted HUVECs showing regulation of HIF targets and proliferation-associated proteins. Cells were generated by lentiviral transduction with FLAG-tagged Cas9, control- (gCtrl) or PHD1/2/3-targeting gRNAs (gPHD1/2/3). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: PHD-2/Egln1

Catalog number: 4835

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Val226 of human PHD-2/Egln1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and PHD1/2/3-depleted HUVECs.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution anti-rabbit HRP-conjugated (Jackson ImmunoResearch Labs, 111-035-008).

References
  1. Andrade J, Shi C, Costa A, Choi J, Kim J, Doddaballapur A, et al. Control of endothelial quiescence by FOXO-regulated metabolites. Nat Cell Biol. 2021;23:413-423 pubmed publisher