This is a knockout-validated antibody summary, based on the publication "Loss of PGRMC1 Delays the Progression of Hepatocellular Carcinoma via Suppression of Pro-Inflammatory Immune Responses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (A) PGRMC1, EGFR, and pEGFR expression levels in DEN-injected livers of WT and Pgrmc1-null mice. (C,D) PGRMC1, EGFR, and pEGFR expression levels in primary hepatocytes (C) and primary non-parenchymal cells (D) from WT and Pgrmc1-null mice.Please see Figure XXXX in the article [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: PGRMC1

Catalog number: 13856

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the amino terminus of human PGRMC1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.

Western blot

WT and Pgrmc1-null mice livers, primary hepatocytes and primary non-parenchymal cells.

Goat anti-Rabbit IgG HRP (Thermo Fisher Scientific, MA, USA, Catalog #31460).

ECL solution (XLS025-0000, Cyanagen, BO, Italy).