This is a knockout-validated antibody summary, based on the publication "UCHL1 regulates muscle fibers and mTORC1 activity in skeletal muscle", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: PGP9.5
Catalog number: ab108986
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human PGP9.5 (UCHL1). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen), immunoprecipitation, and flow cytometry.
Western blot
WT and skeletal muscle UCHL1-KO mice.
3% non-fat milk in PBST solution for 1 h.
Overnight incubation at 4 °C.
Appropriate secondary antibodies conjugated with Alexa-680 or 800 (Invitrogen).
LI-COR scanner (LI-COR Biosciences, Lincoln, NE).
Representative images of Western blots for UCHL1 and GAPDH in soleus, brain and heart from WT-Cre and UCHL1 skmKO mice. Please see Figure 2a in the article [1].
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