PGC-1 antibody | knockout validation | Millipore ST1202-1SET
DOI
//dx.doi.org/10.13070/ko.en.7.2191
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Adult Conditional Knockout of PGC-1α Leads to Loss of Dopamine Neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PGC-1 antibody | knockout validation | Millipore ST1202-1SET figure 1
Figure 1. Immunoblots of PGC-1α and β-actin (loading control) 4 weeks after stereotactic delivery of AAV-GFP and AAV-Cre-GFP into PGC-1αflox/flox mice; n = 3/group. From [1].
Antibody information

Mouse monoclonal IgG

Company: Millipore

Antibody: PGC-1

Catalog number: ST1202-1SET

Summary: Mouse monoclonal IgG against a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α. Reacts with mouse, human and rat. Suitable for immunoblotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Brain cell lysates from control (AAV-GFP delivered into PGC-1αflox/flox) and PGC-1α-KO (AAV-Cre-GFP delivered into PGC-1αflox/flox) mice.

References
  1. Jiang H, Kang S, Zhang S, Karuppagounder S, Xu J, Lee Y, et al. Adult Conditional Knockout of PGC-1α Leads to Loss of Dopamine Neurons. Eneuro. 2016;3: pubmed publisher