PGAM5 antibody | knockout validation | Abcam ab126534
DOI
//dx.doi.org/10.13070/ko.en.7.1939
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "Genetic deficiency of the mitochondrial protein PGAM5 causes a Parkinson's-like movement disorder", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal IgG

Company: Abcam

Antibody: PGAM5

Catalog number: ab126534

Summary: Rabbit polyclonal IgG against an antigen corresponding to amino acids 61-146 of human PGAM5. Reacts with human, mouse, and Xenopus laevis. Suitable for western blot, immunocytochemistry/immunofluorescence and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Mitochondrial extracts of WT and Pgam5 KO MEFs. Mitochondria were purified by using

Qproteome mitochondria isolation Kit (Qiagen) and lysed in 2 x SDS loading buffer.

Primary incubation

1:1,000 dilution in 5% non-fat milk.

Secondary incubation

1:4,000 dilution HRP-labeled secondary antibodies (Southern Biotech).

Figure

Please see Figure 1f in the article [1].

References
  1. Lu W, Karuppagounder S, Springer D, Allen M, Zheng L, Chao B, et al. Genetic deficiency of the mitochondrial protein PGAM5 causes a Parkinson's-like movement disorder. Nat Commun. 2014;5:4930 pubmed publisher