PFKFB3 antibody | knockout validation | Abcam ab181861

This is a knockout-validated antibody summary, based on the publication "Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PFKFB3 antibody | knockout validation | Abcam ab181861 figure 1
Figure 1. Verification of PFKFB3 knockout in HeLa cells by immunoblotting. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: PFKFB3

Catalog number: ab181861

Summary: Rabbit monoclonal IgG against a recombinant fragment within Human PFKFB3 aa 300 to the C-terminus. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Validation Method

Western blot

Sample

Control and PFKFB3 knockout in HeLa cells. Cells were lysed in ice-cold NP-40 buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% NP-40, 1 μg/ml Aprotinin, 1 μg/ml Leupeptin, 1 μg/ml Pepstatin, 1 mM Na3VO4, 50 mM NaF and 1 mM phenylmethylsulfonyl fluoride.

Primary incubation

1:3,000 dilution.

References
  1. Li F, Liu J, Bao R, Yan G, Feng X, Xu Y, et al. Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis. Nat Commun. 2018;9:508 pubmed publisher