This is a knockout-validated antibody summary, based on the publication "Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. The protein expression levels of PEPT1 and β-actin in WT iPSC-IECs and PEPT1-KO iPSC-IECs were examined by western blotting analysis. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: PEPT1

Catalog number: sc-373742

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 366-600 of human PEPT1. Recommended for detection of PEPT1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and PEPT1-KO iPSC--derived intestinal epithelial-like cells (IECs). The cells were homogenized with RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific) containing a protease inhibitor mixture (Thermo Fisher Scientific).

1% skim milk in TBS containing 0.1% Tween 20 at room temperature for 1 h.

At 4°C overnight.

Horseradish peroxidase (HRP)-linked secondary antibodies (Cell Signaling Technology) at room temperature for 1 h.

Chemi-Lumi One Super (Nakalai Tesque) and the signals were read using an LAS-3000 imaging system (Fujifilm).