This is a knockout-validated antibody summary, based on the publication "Functionally specialized junctions between endothelial cells of lymphatic vessels", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Hamster monoclonal

Company: Merck Millipore (Chemicon)

Antibody: PECAM-1

Catalog number: MAB 1398Z, Clone 2H8

Summary: Hamster monoclonal antibody raised against the extracellular domain of mouse PECAM-1. Supplier recommended for electron microscopy, ICC, IHC and IP. Reacts with mouse and dog PECAM-1.

Immunohistochemistry

Lymphatics and blood vessels from wild-type and PECAM-1 -/- mice.

Tissues fixed with 1% paraformaldehyde.

PBS containing 0.3% Tritin X-100, 0.2% BSA and 5% normal goat serum.

Anti-PECAM-1 at 1:500 dilution.

Zeiss LSM-510 confocal microscope with AIM confocal software.

See Figure 4 from the article [1].

The same clone (2H8) is sold as LifeSpan Biosciences LS-C43526; Invitrogen MA3105; Developmental Studies Hybridoma Bank 2H8; EMD Millipore MAB1398Z.