PDPK1 antibody | knockout validation | Santa Cruz sc-17765

This is a knockout-validated antibody summary, based on the publication "Copper Promotes Tumorigenesis by Activating the PDK1-AKT Oncogenic Pathway in a Copper Transporter 1 Dependent Manner", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PDPK1 antibody | knockout validation | Santa Cruz sc-17765 figure 1
Figure 1. PDK1-/- and parental DLD1 cells were stimulated with a time course of copper (20 M) after serum starvation before harvested for IB analysis. From [1].
Antibody information

Mouse monoclonal IgG2a κ

Company: Santa Cruz

Antibody: PDPK1

Catalog number: sc-17765

Summary: Mouse monoclonal antibody against amino acids 229-556 of human PDPK1 (PDK1). Recommended for detection of PDPK1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

WT and PDK1-/- DLD1 cells.

Blocking agent

TBST buffer with 5% non‐fat milk.

Primary incubation

1:1,000 dilution in blocking buffer.

Secondary incubation

Peroxidase‐conjugated anti‐mouse secondary antibody (Sigma, A‐4416).

References
  1. Guo J, Cheng J, Zheng N, Zhang X, Dai X, Zhang L, et al. Copper Promotes Tumorigenesis by Activating the PDK1-AKT Oncogenic Pathway in a Copper Transporter 1 Dependent Manner. Adv Sci (Weinh). 2021;8:e2004303 pubmed publisher