This is a knockout-validated antibody summary, based on publications "PDGFRα/PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes", as cited below [1], "Dysregulated mesenchymal PDGFR-β drives kidney fibrosis" for immunohistochemistry knockout validation (figure ev5b) [2], and "Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms" for immunohistochemistry knockout validation (figure e1f) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: PDGFR beta
Catalog number: ab32570
Summary: Rabbit monoclonal IgG against a synthetic peptide within human PDGFR beta (aa 1,050 to the C-terminus). Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin, frozen and free-float), immunofluorescence/immunocytochemistry, immunoprecipitation and flow cytometry.
Western blot
Control and Pdgfrb knockout immortalized brown preadipocytes.
1:5,000 dilution.
The signal was detected using the Odyssey CLx imaging system (LI-COR).
Knockout of PDGFRβ is confirmed by immunoblots prior to adipogenesis induction. Please see Figure 5b in the article [1].
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