This is a knockout-validated antibody summary, based on the publications "PDGFRα/PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes", as cited below [1], and "Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo" for western blot knockout validation (figure 3a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: PDGF Receptor α
Catalog number: 3174
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy-terminal sequence of human PDGFRα. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.
Western blot
Control and Pdgfra knockout immortalized brown preadipocytes.
1:5,000 dilution.
The signal was detected using the Odyssey CLx imaging system (LI-COR).
Knockout of PDGFRα is confirmed by immunoblot prior to adipogenesis induction. Please see Figure 5a in the article [1].
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