This is a knockout-validated antibody summary, based on the publications "A fast method for analyzing essential protein mutants in human cells" [1] (see below) and "Hepatocyte-Specific β-Catenin Deletion During Severe Liver Injury Provokes Cholangiocytes to Differentiate Into Hepatocytes" for western blot knockout validation (see [2] figure 4a). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: PCNA

Catalog number: sc-56

Summary: Mouse monoclonal IgG2a (kappa light chain) against rat PCNA made in the protein A expression vector pR1T2T. Recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Control cells and PCNA knock-out recovered cells HEK293T.

Western blot of total cell extracts either from control HEK293T cells (1) or from PCNA knock-out compensated cells (2). Please see Figure 1d in the article [1].

The same clone (PC10) is sold as Bio-Rad MCA1558, MCA1558F, MCA1558P; BioLegend 307901, 307902, 307904, 307908, 307909, 307912, 307914; Novus Biologicals NB500-106, NBP2-80905; OriGene TA309795; Santa Cruz Biotechnology sc-56, sc-56 AC, sc-56 HRP; LifeSpan Biosciences LS-C9535, LS-C43736, LS-B1887, LS-C95343, LS-C86889, LS-C86890, LS-C87459, LS-C87502, LS-C87503, LS-B3434, LS-C170844, LS-C180560, LS-C180561, LS-C180563, LS-B9987, LS-C358014; Abnova MAB12946; Enzo Life Sciences ALX-801-091-L001; Abcam ab29; Invitrogen MA5-11355, MA5-11358, PCNA01, 13-3900, 13-3940; GeneTex GTX20029; MyBioSource MBS245134; Absolute Antibody Ab00605-2.0, Ab00605-23.0; Cell Signaling Technology 2586, 8580; Dako M0879; Acris Antibodies SM1421P; Exbio 11-182-C100, 1P-182-T025, 1P-182-T100; BD Biosciences 555567, 555566; EMD Millipore MAB424R, CBL407, MAB424, 05-347, MABE288, NA03.