This is a knockout-validated antibody summary, based on the publication "Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of PCM1 protein levels in PCM1 control (sgCTL) and PCM1 KO RPE1 cells 72 hr after infection with control or Myc-mPCM1 lentivirus. From [1]

Rabbit polyclonal IgG

Company: Santa Cruz

Antibody: PCM1

Catalog number: sc-67204

Summary: Rabbit polyclonal IgG against the epitope corresponding to amino acids 1-262 mapping at the N-terminus of PCM1 of human origin. Recommended for detection of PCM1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, canine and bovine.

Western blot | Immunocytochemistry

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Figure 2. Immunocytochemistry of PCM1 (green) and glutamylated tubulin (GT335, red) of control and PCM1 KO RPE1 cells infected with empty or Myc-mPCM1 lentivirus for 72 hr, serum starved for 48 h. Nuclei were stained with DAPI (blue). Representative images are shown. Scale bar, 5 µm. From [1].

Control and PCM1 KO hTERT RPE-1 cells.

IC: 3% BSA-0.3% PBST (Triton X-100) for 20 min.

IC: diluted in 0.1% PBS with Triton X-100 with 3% BSA for 2 hrs at RT.

IC: diluted in 0.1% PBS with Triton X-100 with 3% BSA for 2 hrs at RT.

IC: 63× or 100×, NA 1.4; Axiovert 200M, Carl Zeiss, Germany) equipped with a cooled CCD (Retiga 2000R; QImaging, Surrey, Canada) and MetaMorph.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.