This is a knockout-validated antibody summary, based on the publication "Identification of phosphoenolpyruvate carboxykinase 1 as a potential therapeutic target for pancreatic cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. The single stable primary pancreatic cancer cells (“pPC1”), bearing the lentiviral CRISPR/Cas9-PCK1-KO construct (“ko-PCK1”) or the CRISPR/Cas9 control empty vector (“Cas9-C”), as well as the parental control cells (“Pare”), were established; Expression of listed proteins were tested. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: PCK1/PEPC

Catalog number: ab133603

Summary: Rabbit monoclonal antibody against a synthetic peptide within human PCK1/PEPC (aa 1-100). Reacts with human. Suitable for western blot and immunohistochemistry (paraffin). Unsuitable for immunocytochemistry, immunoprecipitation or flow cytometry.

Western blot

Parental and PCK1-KO primary pancreatic cancer cells.

1:1,000 dilution.