This is a knockout-validated antibody summary, based on the publication "Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis demonstrated the loss of Pcgf1 protein in ES cells. Pcgf1 was detected as a 30 kDa band in WT extracts but not in extracts from Pcgf1−/− ES cells. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: PCGF1

Catalog number: sc-515371

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 128-247 mapping near the C-terminus of human PCGF1. Recommended for detection of PCGF1 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and Pcgf1−/− ES cells. Cells were harvested and lysed in hypotonic lysis buffer (10 mM Tris–Cl pH 8.0, 1 mM KCl, 1.5 mM MgCl2 and 0.5mM β-mercaptoethanol, 10 mg/ml PMSF, Protease Inhibitor Mix (Sigma)).

5% (w/v) non-fat milk for one hour at room temperature.

1:1,000 dilution overnight at 4 °C.

1:5,000 dilution horseradish peroxidase-conjugated goat anti-mouse lgG-HRP (sc-2005, Santa Cruz Biotechnology).

ECL blot detection system.