This is a knockout-validated antibody summary, based on the publication "Multiple protocadherins are expressed in brain microvascular endothelial cells and might play a role in tight junction protein regulation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgA (kappa light chain)

Company: Santa Cruz

Antibody: PCDHGC

Catalog number: sc-130556

Summary: Mouse monoclonal IgA (kappa light chain) against a 124 amino acid sequence corresponding to the conserved C-terminal region of mouse PCDHGC. Recommended for detection of proteins containing the conserved C-terminal region of PCDHGC of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Wild-type cerebEND (wt) and PcdhgC3 knockout cerebEND (KO) cells. Cells were lysed in RIPA buffer supplemented with protease inhibitors cocktail (Roche).

10% (w/v) low fat milk in PBS.

Diluted in blocking solution overnight at 4C.

Horseradish peroxidase-labeled anti-mouse IgA (Santa Cruz Biotechnology).

Enhanced chemiluminescence detection kit (Promega) and FluorChem FC2 Multi-imager II (Alpha Innotech) with CD camera.

Efficiency of PcdhgC3 knockout in cerebEND. b-actin served as a loading control. The C-terminal fragment of PcdhgC3 of about 25 kDa in wild-type cerebEND is indicated by the arrow. Please see Figure 3a in the article [1].

PcdhgC3-Biotin labeled antibody (Stress Marq) was the one used for IC (Fig. 4b).