PCDH1 antibody | knockout validation

PCDH1 antibody | knockout validation | Santa Cruz sc-81816

This is a knockout-validated antibody summary, based on the publication "Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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PCDH1 antibody | knockout validation | Santa Cruz sc-81816 figure 1

Figure 1. Western blot analysis of WT TIME cells and KO cells ± cDNA. b-Actin was used as a loading control. From [1].

Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: PCDH1

Catalog number: sc-81816

Summary: Mouse monoclonal antibody against recombinant human PCDH1. Recommended for detection of PCDH1 of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and ELISA.

Validation Method

Western blot

Sample

WT and PCDH1 KO TIME cells.

Primary incubation

1:200 dilution.

Secondary incubation

1:10,000 dilution IRDye 680LT Goat α-Mouse secondaries Abs (LI-COR).

Detection

LI-COR Fc fluorescent imager.

References

Dieterle M, Solà Riera C, Ye C, Goodfellow S, Mittler E, Kasikci E, et al. Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells. elife. 2021;10: pubmed publisher

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