PBEF/NAMPT antibody | knockout validation | Cell Signaling 61122

This is a knockout-validated antibody summary, based on the publication "Nuclear NAD + homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PBEF/NAMPT antibody | knockout validation | Cell Signaling 61122 figure 1
Figure 1. Immunoblot of NAMPT in MOLM13 cells at day 4 after deleting the gene. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: PBEF/NAMPT

Catalog number: 61122

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gly122 of human PBEF/NAMPT protein. Reacts with human by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and NAMPT KO MOLM13 cells.

Blocking agent

5% (w/v) nonfat milk in 1× PBST (PBS with 0.1% Tween-20) for 1 hour at room temperature.

Primary incubation

1:1,000 dilution overnight at 4°C.

Secondary incubation

1:5,000-10,000 dilution anti-rabbit horseradish peroxidase–conjugated secondary antibodies (Cell Signaling Technology).

Detection

SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, catalog no. 34580) and a KODAK X-OMAT 2000 Processor (Kodak).

References
  1. Shi X, Jiang Y, Kitano A, Hu T, Murdaugh R, Li Y, et al. Nuclear NAD+ homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells. Sci Adv. 2021;7: pubmed publisher