This is a knockout-validated antibody summary, based on the publication "Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (upper panel) Western blot showing the absence of the PAXX protein in Paxx-deleted pro B cell clones. From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: PAXX

Catalog number: ab126353

Summary: Rabbit polyclonal IgG against a synthetic peptide corresponding to amino acids 109-204 of human C9orf142. Reacts with human by western blot, immunocytochemistry/immunofluorescence and immunohistochemistry.

Western blot

WT and Paxx knockout v-abl pro-B cells. Cells were lysed using RIPA cell lysis reagent (Thermo Fisher Scientific) and protease inhibitors cocktail (Sigma-Aldrich).

5% non-fat dried milk in TBS containing 0.05% Tween-20 buffer for 1 hr at room temperature.

1:1,000 dilution overnight at 4°C.

1:20,000 dilution HRP-conjugated antibodies (#7074, Cell Signaling Technology) for 1 hr at room temperature.

WesternBright Sirius substrate (Advansta).